Category: bioinformatics

Bioinformatics is a rapidly evolving field, and staying updated with the latest trends, tools, and metrics is crucial. Below is…

Converting GFF3 (General Feature Format version 3) to GTF (Gene Transfer Format) is a common task in bioinformatics, especially for…

SNP calling has evolved with the introduction of new tools and improvements in existing ones. Below is an updated workflow…

Selecting the right pipeline framework is critical for ensuring scalability, reproducibility, and ease of use in bioinformatics projects. Below is…

Developing good habits as a bioinformatics analyst or scientist is essential for ensuring efficiency, reproducibility, and collaboration. Below is a…

1. Define a Clear Directory Structure Create a standardized hierarchy for your projects to avoid confusion and ensure consistency. Here’s…

Filtering a multi-FASTA file by sequence length is a common task in bioinformatics. Below are step-by-step instructions using Unix, Perl,…

SNP Calling refers to the process of identifying single nucleotide polymorphisms (SNPs) in sequencing data, distinguishing genuine variations from sequencing…

This guide provides instructions to set up, explore, and visualize SAM/BAM files using tools like samtools, IGV, and Tablet, as…

This guide will clarify the differences between FPKM, raw counts, and RPKM in RNA-seq analysis, explaining when and how to…

This step-by-step guide will help you comprehend the output of samtools view. The guide covers essential details about the fields…

Differential Expression (DE) refers to the process of identifying and analyzing genes whose expression levels vary significantly between different biological…

Analyzing imputed GWAS data involves several steps, including quality control (QC), association analysis, and interpretation of results. Below is a…

Introduction FASTA files (.fasta, .fa, .fsa) are commonly used in bioinformatics to store nucleotide or protein sequences. The .fa extension is just a shorthand…

Install Local BLAST in Ubuntu Step 1: Update Your System Before installing any software, ensure your system is up-to-date: bash…

To convert chemical SMILES strings to IUPAC names, several open-source and freely available tools can be used. Below is a…