Category: bioinformatics

Introduction to Minor Allele Frequency Definition: Minor allele frequency (MAF) is the proportion of the second most common allele (the…

ntroduction Counting sequences in a FASTQ.GZ file is a common task in bioinformatics for verifying data integrity, ensuring proper preprocessing,…

Heatmaps are essential tools for visualizing complex data, such as gene expression, in an intuitive and comprehensible manner. They provide…

This guide is designed to help beginners understand Synonymous and Non-Synonymous Single Nucleotide Polymorphisms (SNPs), how to distinguish between them,…

Introduction: In bioinformatics, BAM files are essential for storing aligned sequencing data, typically in the Binary Alignment/Map format. Sorting BAM…

1. Introduction: In bioinformatics, when performing read alignment (typically with tools like BWA, Bowtie, or HISAT2), we encounter two common…

Converting Affymetrix probes to gene IDs is an essential step in analyzing gene expression data derived from Affymetrix microarrays. It…

Introduction A comprehensive database of tumor suppressors and oncogenes is a crucial resource for cancer research. These databases provide insights…

Converting SAM (Sequence Alignment/Map) files to BAM (Binary Alignment/Map) format is a crucial task in bioinformatics, particularly in next-generation sequencing…

VCF (Variant Call Format) files are widely used in bioinformatics to store genomic data, particularly for representing variant information such…

Microarray data analysis is an essential task in bioinformatics, often used to examine gene expression patterns. Bioconductor, an open-source software…

Whole Exome Sequencing (WES) is a powerful tool for understanding genetic variations within coding regions of the genome. This pipeline…

Introduction to Coverage Calculation Coverage, in the context of genome sequencing, refers to how many times a particular base or…

This guide aims to help you evaluate whether pursuing a Master’s in Bioinformatics (MSc) will benefit your career prospects, especially…

Comprehensive Guide to the pheatmap Package in R Introduction to pheatmap The pheatmap package in R is a versatile and…

Here’s a step-by-step guide for beginner bioinformatics students to embark on a project. This guide combines the ideas from the…

Here is a comprehensive step-by-step manual for combining FASTA files using both Unix/Linux and Windows approaches. This guide includes recent…

This guide will help you remove duplicate sequences from a FASTA file using various methods, including Unix commands, Perl, and…

Step-by-Step Guide: Calculating Sequence Lengths from a FASTA File This guide explains various methods to calculate sequence lengths from a…