Bioinformatics, AI, Genomics, Proteomics and Transcriptomics
1. Introduction: In bioinformatics, when performing read alignment (typically with tools like BWA, Bowtie, or HISAT2), we encounter two common…
Converting Affymetrix probes to gene IDs is an essential step in analyzing gene expression data derived from Affymetrix microarrays. It…
Introduction A comprehensive database of tumor suppressors and oncogenes is a crucial resource for cancer research. These databases provide insights…
Converting SAM (Sequence Alignment/Map) files to BAM (Binary Alignment/Map) format is a crucial task in bioinformatics, particularly in next-generation sequencing…
VCF (Variant Call Format) files are widely used in bioinformatics to store genomic data, particularly for representing variant information such…
Microarray data analysis is an essential task in bioinformatics, often used to examine gene expression patterns. Bioconductor, an open-source software…
Whole Exome Sequencing (WES) is a powerful tool for understanding genetic variations within coding regions of the genome. This pipeline…
Introduction to Coverage Calculation Coverage, in the context of genome sequencing, refers to how many times a particular base or…
This guide aims to help you evaluate whether pursuing a Master’s in Bioinformatics (MSc) will benefit your career prospects, especially…
Comprehensive Guide to the pheatmap Package in R Introduction to pheatmap The pheatmap package in R is a versatile and…
Here’s a step-by-step guide for beginner bioinformatics students to embark on a project. This guide combines the ideas from the…
As a bioinformatician, you may be tasked with explaining the differences between various methods for differential expression (DE) analysis, such…
Here is a comprehensive step-by-step manual for combining FASTA files using both Unix/Linux and Windows approaches. This guide includes recent…
This guide will help you remove duplicate sequences from a FASTA file using various methods, including Unix commands, Perl, and…
Step-by-Step Guide: Calculating Sequence Lengths from a FASTA File This guide explains various methods to calculate sequence lengths from a…
Here’s a step-by-step manual on how to extract FASTA sequences from a file using a list of headers provided in…
Here’s a step-by-step manual to extract user-defined regions from a FASTA file. This guide incorporates both UNIX commands and Perl…
Understanding how much sequencing depth is needed for an RNA-Seq experiment depends on your experimental goals, sample complexity, and the…
Here is a step-by-step guide for BAM/SAM to FASTA conversion, tailored to beginners and incorporating the latest tools and techniques:…
This guide provides a beginner-friendly manual to determine the sequence length distribution of reads in a FASTQ file. It incorporates…