Category: Guides

If you are looking for mutation databases specialized in cancer, particularly for breast cancer, there are several well-curated resources available.…

Gene symbols like SEPT1, MARCH1, and APR1 are often automatically converted into date formats (e.g., 1-Sep, 1-Mar, 1-Apr) when opened in Microsoft Excel. This can lead to…

Indexing a genome is a crucial step in bioinformatics, especially when working with tools like Bowtie, Bowtie2, or other sequence…

In this guide, we will explore various databases and resources that provide information on patented drugs and compounds. These resources…

In this guide, we will walk through the process of calculating Transcripts Per Million (TPM) from the output of featureCounts. TPM…

In this guide, we will walk through the process of adding gene names to a volcano plot generated from DESeq2…

This step-by-step guide will walk you through a basic RNA-seq data analysis workflow using edgeR in R. The workflow includes data loading,…

Explaining bioinformatics to a 10-year-old can be both fun and challenging. The key is to use simple, relatable concepts and…

The CIGAR (Compact Idiosyncratic Gapped Alignment Report) string in SAM/BAM files is a crucial component for representing sequence alignments. However,…

Counting the number of sequences in a FASTQ file is a common task in bioinformatics. When dealing with compressed FASTQ…

Parallel computing is essential in bioinformatics due to the large volumes of data and computationally intensive tasks. This guide provides…

RNA-Seq data coverage is a critical metric for assessing the quality and depth of sequencing experiments. Unlike DNA sequencing, RNA-Seq…

Bioinformatics is an interdisciplinary field that combines biology, computer science, mathematics, and statistics to analyze and interpret biological data. This…

Determining an appropriate threshold for log2 fold change (log2FC) is a critical step in identifying differentially expressed genes (DEGs) in…

Trimming Illumina reads is a crucial step in preprocessing sequencing data to remove low-quality bases, adapter sequences, and other artifacts.…

Finding common motifs in sequences is a fundamental task in bioinformatics, particularly for identifying conserved regions in DNA, RNA, or…

Running bioinformatics tools like Picard can sometimes lead to “out of disk space” errors, even when you have ample storage.…

Communicating complex bioinformatics concepts to non-computational biologists (NCBs) can be challenging. This guide provides practical tips and strategies to bridge…