Category: Guides

Counting the number of N characters (ambiguous bases) in FASTA sequences is a common task in bioinformatics. Below are several methods to…

Extracting specific sequences from a large FASTA file is a common task in bioinformatics. Below are several methods to achieve…

Bioinformatics is a field that combines biology, computer science, and statistics, and while it is incredibly rewarding, it comes with…

Entering the field of bioinformatics can be both exciting and overwhelming. Here are some key insights, tips, and advice that…

Bioinformatics is a highly interdisciplinary field, and the tools and techniques you need to master depend on your specific area…

1. Understanding Paired-End Sequencing Definition: Paired-end sequencing involves sequencing both ends of a DNA fragment, providing two reads (forward and reverse)…

Bioinformatics is a rapidly evolving field, and staying updated with the latest trends, tools, and metrics is crucial. Below is…

Converting GFF3 (General Feature Format version 3) to GTF (Gene Transfer Format) is a common task in bioinformatics, especially for…

SNP calling has evolved with the introduction of new tools and improvements in existing ones. Below is an updated workflow…

Selecting the right pipeline framework is critical for ensuring scalability, reproducibility, and ease of use in bioinformatics projects. Below is…

Developing good habits as a bioinformatics analyst or scientist is essential for ensuring efficiency, reproducibility, and collaboration. Below is a…

1. Define a Clear Directory Structure Create a standardized hierarchy for your projects to avoid confusion and ensure consistency. Here’s…

Filtering a multi-FASTA file by sequence length is a common task in bioinformatics. Below are step-by-step instructions using Unix, Perl,…

SNP Calling refers to the process of identifying single nucleotide polymorphisms (SNPs) in sequencing data, distinguishing genuine variations from sequencing…