January 9, 2025
Step-by-Step Guide to Understanding Different Bioinformatics Positions
Bioinformatics is a multidisciplinary field that combines biology, computer science, and statistics. As the field grows, so do the variety…
Category: Guides
January 9, 2025
Step-by-Step Manual to Find Disease-Associated SNPs
This guide provides the latest tools, databases, and tips to identify disease-associated SNPs, including associated PMIDs, case/control numbers, and population…
January 9, 2025
Step-by-Step Manual: How Bioinformatics Has Improved Over Time
Bioinformatics has evolved significantly over the past few decades, driven by advancements in sequencing technologies, computational power, and the development…
January 9, 2025
Step-by-Step Manual: Numbers Every Bioinformatician Should Know
Bioinformatics is a rapidly evolving field, and staying updated with the latest trends, tools, and metrics is crucial. Below is…
January 9, 2025
Step-by-Step Manual: Merging Many Small BAM Files into One Large BAM File
Merging thousands of small BAM files into a single large BAM file is a common task in bioinformatics, especially for…
January 9, 2025
Step-by-Step Manual: Converting GFF3 to GTF
Converting GFF3 (General Feature Format version 3) to GTF (Gene Transfer Format) is a common task in bioinformatics, especially for…
January 9, 2025
Step-by-Step Manual: SNP Calling Workflow
SNP calling has evolved with the introduction of new tools and improvements in existing ones. Below is an updated workflow…
January 9, 2025
Step-by-Step Manual: Choosing a Bioinformatics-Friendly Pipeline Building Framework
Selecting the right pipeline framework is critical for ensuring scalability, reproducibility, and ease of use in bioinformatics projects. Below is…
January 9, 2025
Step-by-Step Manual: Good Habits for Bioinformatics Analysts or Scientists
Developing good habits as a bioinformatics analyst or scientist is essential for ensuring efficiency, reproducibility, and collaboration. Below is a…
January 9, 2025
Step-by-Step Manual: How to Manage Your Files & Directories for Bioinformatics Projects
1. Define a Clear Directory Structure Create a standardized hierarchy for your projects to avoid confusion and ensure consistency. Here’s…
January 3, 2025
How To Filter Multi-FASTA By Length?
Filtering a multi-FASTA file by sequence length is a common task in bioinformatics. Below are step-by-step instructions using Unix, Perl,…
January 3, 2025
SNP Calling: A Step-by-Step Guide
SNP Calling refers to the process of identifying single nucleotide polymorphisms (SNPs) in sequencing data, distinguishing genuine variations from sequencing…
January 3, 2025
Step-by-Step Instructions: Exploring and Visualizing SAM/BAM Files
This guide provides instructions to set up, explore, and visualize SAM/BAM files using tools like samtools, IGV, and Tablet, as…
January 3, 2025
FPKM vs Raw Counts vs RPKM: Step-by-Step Guide
This guide will clarify the differences between FPKM, raw counts, and RPKM in RNA-seq analysis, explaining when and how to…
January 3, 2025
Understanding Samtools View Output
This step-by-step guide will help you comprehend the output of samtools view. The guide covers essential details about the fields…
January 3, 2025
What is Differential Expression?
Differential Expression (DE) refers to the process of identifying and analyzing genes whose expression levels vary significantly between different biological…
January 3, 2025
How To Analyze Imputed GWAS Data
Analyzing imputed GWAS data involves several steps, including quality control (QC), association analysis, and interpretation of results. Below is a…
January 3, 2025
Fasta File Vs Fa File: Merging and Conversion
Introduction FASTA files (.fasta, .fa, .fsa) are commonly used in bioinformatics to store nucleotide or protein sequences. The .fa extension is just a shorthand…
January 3, 2025
Step-by-Step Guide: Install Local BLAST in Ubuntu
Install Local BLAST in Ubuntu Step 1: Update Your System Before installing any software, ensure your system is up-to-date: bash…
January 3, 2025
Converting Chemical SMILES to IUPAC Names: Open Source Tools and Scripts
To convert chemical SMILES strings to IUPAC names, several open-source and freely available tools can be used. Below is a…