Step-by-Step Guide: using the pheatmap package in R to annotate heatmaps
Comprehensive Guide to the pheatmap Package in R Introduction to pheatmap The pheatmap package in R is a versatile and…
Bioinformatics, AI, Genomics, Proteomics and Transcriptomics
Comprehensive Guide to the pheatmap Package in R Introduction to pheatmap The pheatmap package in R is a versatile and…
Here’s a step-by-step guide for beginner bioinformatics students to embark on a project. This guide combines the ideas from the…
As a bioinformatician, you may be tasked with explaining the differences between various methods for differential expression (DE) analysis, such…
Here is a comprehensive step-by-step manual for combining FASTA files using both Unix/Linux and Windows approaches. This guide includes recent…
This guide will help you remove duplicate sequences from a FASTA file using various methods, including Unix commands, Perl, and…
Step-by-Step Guide: Calculating Sequence Lengths from a FASTA File This guide explains various methods to calculate sequence lengths from a…
Here’s a step-by-step manual on how to extract FASTA sequences from a file using a list of headers provided in…
Here’s a step-by-step manual to extract user-defined regions from a FASTA file. This guide incorporates both UNIX commands and Perl…
Understanding how much sequencing depth is needed for an RNA-Seq experiment depends on your experimental goals, sample complexity, and the…
Here is a step-by-step guide for BAM/SAM to FASTA conversion, tailored to beginners and incorporating the latest tools and techniques:…
This guide provides a beginner-friendly manual to determine the sequence length distribution of reads in a FASTQ file. It incorporates…
This guide explains the fundamentals of algorithms, their relevance in bioinformatics, and a detailed step-by-step approach to creating an algorithm…
When working with many FASTQ files, such as those derived from sequencing runs of the same sample, merging them is…
Introduction FASTQ and FASTA are standard formats in bioinformatics. Sometimes, it’s necessary to convert multiline FASTA sequences to a single-line…
This manual explains how to convert FASTQ files to FASTA format while ensuring compatibility across systems. Several methods are provided,…
Extracting reads from a BAM file that fall entirely within a given region. The process ensures you exclude reads overlapping…
This guide provides a detailed explanation of BAM and SAM formats and step-by-step instructions on converting BAM to SAM using…
1. Understand BLAST Output Options BLAST’s -outfmt parameter allows you to control the format and content of the output file.…
This guide outlines the process of downloading raw sequencing data from GEO/SRA using the NCBI SRA Toolkit. We will also…
Bioinformatics is one of the most exciting and rapidly growing fields today, combining biology, computer science, and statistics to unlock…